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More Great Information on Procedure Zone:
Analysis of alpha‐1‐acid glycoprotein isoforms using CE‐LIF with fluorescent thiol derivatization (Med Worm)
The analysis of glycoprotein isoforms is of high interest in the biomedical
field and clinical chemistry. Many studies have demonstrated that some
glycoprotein isoforms could serve as biomarkers for several major diseases,
such as cancers and vascular diseases, among others. Capillary zone
electrophoresis (CZE) is a well‐established technique to separate glycoprotein
isoforms, however, it suffers from limited sensitivity when UV‐Vis detection
is used. On the other hand, with laser‐induced fluorescence (LIF) detection,
derivatization reaction to render the proteins fluorescent can destroy the
resolution of the isoforms. In this work, a derivatization procedure through
the thiol groups of glycoproteins using either 5‐(iodoacetamide) fluorescein
(5‐IAF) or BODIPY iodoacetamide is pr...
Tags: procedure zone, procedure zone electrophoresis, procedure zone inhibition, procedure zone system, procedure zone transfer